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작성자 천재 작성일26-04-02 18:06 댓글36건

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인간 유도만능줄기세포(hiPSC; BJFF.6, 남성, 워싱턴 대학교 Sanjay Jain 교수 제공)는 1% Matrigel이 코팅된 DMEM/F12 배지에서 mTeSR Plus(mTeSR+) 배지를 사용하여 배양했습니다. iPSC는 세포 밀도가 60%~80%에 도달했을 때 ReLeSR(제조사 프로토콜에 따름)을 이용하여 1:8~1:20의 분할 비율로 계대배양했습니다. 냉동 보관된 세포는 배아세포 동결 배지와 1:1 비율의 mTeSR+ 배지에 10 μM Y27632를 첨가하여 보관했습니다. 세포의 정상 핵형은 WiCell을 이용하여 확인했습니다. 최소 6개월마다 Lonza 마이코플라스마 검출 키트를 사용하여 마이코플라스마 검사를 정기적으로 실시했습니다. 핵형 확인 후 iPSC는 15회 이상 계대배양하지 않았습니다.

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